FIG. 3.

Effect of bacteriophage cocktail applied to S. Typhimurium 8243 and S. Typhimurium DT104 in TSB-R at initial concentrations of 10⁸ CFU/ml. S. Typhimurium 8243 (○) and S. Typhimurium DT104 (□) were treated with bacteriophage at time zero (solid black lines), followed by phage treatment at 10 h (broken black lines); control bacteria (not treated with bacteriophage) were treated with SM buffer at time zero (solid gray lines), followed by a second SM buffer treatment at 10 h (broken gray lines). Optical density measurements of control and bacteriophage treatment for the single application of bacteriophage mixture were measured every hour for 9 h. At 10 h, a second round of SM buffer or bacteriophage was added to duplicate samples of controls and treatments, respectively. OD₆₀₀, optical density at 600 nm.