TABLE 1.

Primer pairs used for qRT-PCR analysis

GeneID	Gene name	Protein annotation	Primer typea	Primer sequence
STM1572	nmpC	Putative outer membrane porin precursor	F	5′-CGA CCA GGA TCT GGT TGA GT-3′
			R	5′-CTT TAG CCG CTT TGG TGA AG-3′
STM2543	nifS	Cysteine desulfurase	F	5′-ATC GCG AAA GAA GAG ATG GA-3′
			R	5′-TCG CCG TTC AGG TAA ACT TC-3′
STM2542	nifU	NifU-like protein	F	5′-AAC GAC GAT AAC GTG GGA AG-3′
			R	5′-GCA GCC GTA AGT CTT GAA GC-3′
STM2544	yfhP	Putative iron-sulfur cluster regulatory protein	F	5′-TTA CCT TAG GCG AGC TGG TG-3′
			R	5′-GCG CGT AAT TTA ACG TCG AT-3′
STM0823	ybiJ	Putative periplasmic protein	F	5′-TGC ATA AAA TCG GCG TAG TG-3′
			R	5′-TGC CGC TCA TTT TGT CAT TA-3′
STM1214	ycfR	Putative outer membrane protein	F	5′-ACG CCA GAA GGT CAA CAG AA-3′
			R	5′-GGG CCG GTA ACA GAG GTA A-3′
STM2430	cysK	O-Acetylserine sulfhydrolase A	F	5′-CGC TAT TCA GAA AGC CGA AG-3′
			R	5′-CAT CGG TGT CTT CCC AGA TT-3′
STM2338	pta	Phosphate acetyltransferase	F	5′-AGC CAC GTT GAA TCT CTG CT-3′
			R	5′-AGA CCT TCA ACC AGC ACC AC-3′
STM3867	atpA	ATP synthase subunit A	F	5′-GGC GAC GTA TTC TAC CTC CA-3′
			R	5′-CGG TTT TCC CTT TCA CTT CA-3′
STM1378	pykF	Pyruvate kinase	F	5′-ATG AAC GTG ATG CGT CTG AA-3′
			R	5′-TAA TGG TGC GGA TTT CTG GT-3′
SEN2085D	rfbS	Paratose synthase	F	F:5′-TGGCTTAGCAAGGAAGAGGA-3′
			R	5′-TGGCAGTGATGTTCCACAAT-3′
SEN2085C	rfbE	CDP-tyvelose-2-epimerase	F	F:5′-CAAAAGGTGCTGCAGATCAA-3′
			R	5′-CAGCATGCAAAACATCCCTA-3′

^aF, forward; R, reverse.