FIG. 2.
Effect of oligonucleotide length on RecTPsy-mediated recombination. P. syringae cells containing either the RecTPsy expression vector or the empty vector control were transformed with 1 μg of oligonucleotide. Each oligonucleotide was designed with two equal-length regions of homology (combined lengths are indicated on the x axis) flanking the 4-base change coding for streptomycin resistance. For example, the 40-bp homology oligonucleotide is composed of two 20-nt segments of the rpsL sequence flanking the 4-base change. The oligonucleotides used in this experiment are oSWC1978 (10 nt), oSWC1518 (20 nt), oSWC1251 (40 nt), oSWC1253 (60 nt), oSWC1255 (80 nt), and oSWC1257 (120 nt). See Table 2 for the complete sequence of each oligonucleotide.