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1H NMR spectra of xeroprotectants obtained from 4J27D (A) or 4J27 (B), showing that the extraction/milking protocol influences the xeroprotectant mixture composition. Peak assignments are as follows: 1, alanine; 2, glutamate; 3, glutamine; 4, 6, and 8, glucose; 5 and 7, trehalose. The area between 3.7 and 4 ppm corresponds mainly to protons from glucose and trehalose.
