TABLE 1.
PCR method (purpose), locus, and primera | Primer sequence (5′→3′) | Size of product (bp) | Reference |
---|---|---|---|
M-PCR 1 (for identifying S. aureus and detecting mecA and pvl) | |||
mecA1 | TGT CCG TAA CCT GAA TCA GC | 519 | 47 |
mecA2 | TGC TAT CCA CCC TCA AAC AG | ||
femA-2F | AAC TGT TGG CCA CTA TGA GT | 308 | 35 |
femA-2R | CCA GCA TTA CCT GTA ATC TCG | ||
luk-PV-1 (modified) | TCA TTA GGT AAA ATG TCT GGA CAT GAT CCA | 433 | 20 |
luk-PV-2 | GCA TCA AST GTA TTG GAT AGC AAA AGC | ||
M-PCR 2 (for amplication of mec gene complex class) | |||
mI6 | CAT AAC TTC CCA TTC TGC AGA TG | 1,963 | 18 |
IS7 | ATG CTT AAT GAT AGC ATC CGA ATG | 2,827 | |
IS2 | TGA GGT TAT TCA GAT ATT TCG ATG T | 804 | |
mA7 | ATA TAC CAA ACC CGA CAA CTA CA | ||
M-PCR 3 (for amplification of ccr gene complex type) | |||
ccrAB-β2 | ATT GCC TTG ATA ATA GCC ITC T | ||
ccrAB-α2 | AAC CTA TAT CAT CAA TCA GTA CGT | 695 | 16 |
ccrAB-α3 | TAA AGG CAT CAA TGC ACA AAC ACT | 937 | |
ccrAB-α4 | AGC TCA AAA GCA AGC AAT AGA AT | 1,791 | |
M-PCR 4 (for amplication of elements on SCCmec) | |||
Locus A | |||
CIF2 F2 | TTC GAG TTG CTG ATG AAG AAG G | 495 | 33 |
CIF2 R2 | ATT TAC CAC AAG GAC TAC CAG C | ||
Locus B | |||
KDP F1 | AAT CAT CTG CCA TTG GTG ATG C | 284 | 33 |
KDP R1 | CGA ATG AAG TGA AAG AAA GTG G | ||
Locus C | |||
MECI P2 | ATC AAG ACT TGC ATT CAG GC | 209 | 33 |
MECI P3 | GCG GTT TCA ATT CAC TTG TC | ||
Locus D | |||
DCS F2 | CAT CCT ATG ATA GCT TGG TC | 342 | 33 |
DCS R1 | CTA AAT CAT AGC CAT GAC CG | ||
Locus E | |||
RIF4 F3 | GTG ATT GTT CGA GAT ATG TGG | 243 | 33 |
RIF4 R9 | CGC TTT ATC TGT ATC TAT CGC | ||
Locus F | |||
RIF5 F10 | TTC TTA AGT ACA CGC TGA ATC G | 414 | 33 |
RIF5 R13 | GTC ACA GTA ATT CCA TCA ATG C | ||
Locus G | |||
IS431 P4 | CAG GTC TCT TCA GAT CTA CG | 381 | 33 |
pUB110 R1 | GAG CCA TAA ACA CCA ATA GCC | ||
Locus H | |||
IS431 P4 | CAG GTC TCT TCA GAT CTA CG | 303 | 33 |
pT181 R1 | GAA GAA TGG GGA AAG CTT CAC | ||
mecA | |||
MECA P4 | TCC AGA TTA CAA CTT CAC CAG G | 162 | 33 |
MECA P7 | CCA CTT CAT ATC TTG TAA CG | ||
PCR 1 (for identification of S. pseudintermedius by restriction fragment length polymorphism using MboI) | |||
pta_f1 | AAA GAC AAA CTT TCA GGT AA | 320 | 4 |
pta_r1 | GCA TAA ACA AGC ATT GTA CCG | ||
PCR 2 (for amplification of the ccrC gene) | |||
ccrC F2 | GTA CTC GTT ACA ATG TTT GG | 449 | 27 |
ccrC R2 | ATA ATG GCT TCA TGC TTA CC | ||
PCR 3 (for amplification of the J1 region) | |||
SCCmec III J1 F | CAT TTG TGA AAC ACA GTA CG | 243 | 27 |
SCCmec III J1 R | GTT ATT GAG ACT CCT AAA GC |
Forward and reverse primers are indicated by F and R, respectively.