TABLE 1.

Primers used in this study

PCR method (purpose), locus, and primera	Primer sequence (5′→3′)	Size of product (bp)	Reference
M-PCR 1 (for identifying S. aureus and detecting mecA and pvl)
mecA1	TGT CCG TAA CCT GAA TCA GC	519	47
mecA2	TGC TAT CCA CCC TCA AAC AG
femA-2F	AAC TGT TGG CCA CTA TGA GT	308	35
femA-2R	CCA GCA TTA CCT GTA ATC TCG
luk-PV-1 (modified)	TCA TTA GGT AAA ATG TCT GGA CAT GAT CCA	433	20
luk-PV-2	GCA TCA AST GTA TTG GAT AGC AAA AGC
M-PCR 2 (for amplication of mec gene complex class)
mI6	CAT AAC TTC CCA TTC TGC AGA TG	1,963	18
IS7	ATG CTT AAT GAT AGC ATC CGA ATG	2,827
IS2	TGA GGT TAT TCA GAT ATT TCG ATG T	804
mA7	ATA TAC CAA ACC CGA CAA CTA CA
M-PCR 3 (for amplification of ccr gene complex type)
ccrAB-β2	ATT GCC TTG ATA ATA GCC ITC T
ccrAB-α2	AAC CTA TAT CAT CAA TCA GTA CGT	695	16
ccrAB-α3	TAA AGG CAT CAA TGC ACA AAC ACT	937
ccrAB-α4	AGC TCA AAA GCA AGC AAT AGA AT	1,791
M-PCR 4 (for amplication of elements on SCCmec)
Locus A
CIF2 F2	TTC GAG TTG CTG ATG AAG AAG G	495	33
CIF2 R2	ATT TAC CAC AAG GAC TAC CAG C
Locus B
KDP F1	AAT CAT CTG CCA TTG GTG ATG C	284	33
KDP R1	CGA ATG AAG TGA AAG AAA GTG G
Locus C
MECI P2	ATC AAG ACT TGC ATT CAG GC	209	33
MECI P3	GCG GTT TCA ATT CAC TTG TC
Locus D
DCS F2	CAT CCT ATG ATA GCT TGG TC	342	33
DCS R1	CTA AAT CAT AGC CAT GAC CG
Locus E
RIF4 F3	GTG ATT GTT CGA GAT ATG TGG	243	33
RIF4 R9	CGC TTT ATC TGT ATC TAT CGC
Locus F
RIF5 F10	TTC TTA AGT ACA CGC TGA ATC G	414	33
RIF5 R13	GTC ACA GTA ATT CCA TCA ATG C
Locus G
IS431 P4	CAG GTC TCT TCA GAT CTA CG	381	33
pUB110 R1	GAG CCA TAA ACA CCA ATA GCC
Locus H
IS431 P4	CAG GTC TCT TCA GAT CTA CG	303	33
pT181 R1	GAA GAA TGG GGA AAG CTT CAC
mecA
MECA P4	TCC AGA TTA CAA CTT CAC CAG G	162	33
MECA P7	CCA CTT CAT ATC TTG TAA CG
PCR 1 (for identification of S. pseudintermedius by restriction fragment length polymorphism using MboI)
pta_f1	AAA GAC AAA CTT TCA GGT AA	320	4
pta_r1	GCA TAA ACA AGC ATT GTA CCG
PCR 2 (for amplification of the ccrC gene)
ccrC F2	GTA CTC GTT ACA ATG TTT GG	449	27
ccrC R2	ATA ATG GCT TCA TGC TTA CC
PCR 3 (for amplification of the J1 region)
SCCmec III J1 F	CAT TTG TGA AAC ACA GTA CG	243	27
SCCmec III J1 R	GTT ATT GAG ACT CCT AAA GC

^aForward and reverse primers are indicated by F and R, respectively.