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Enzymatic parameters of 3Dpol elongation complexes
| 3Dpol | RNA Kd (μM) | Elongation rate Vmax (nt/s) | NTP Km (μM) | Elongation complex stability (min) |
|---|---|---|---|---|
| Wild type | 8.6 ± 0.6 | 2.1 ± 0.1 | 18 ± 3 | 186 ± 4 |
| Trp5Tyr mutant | NDa | ND | ND | 56 ± 3 |
| Trp5Phe mutant | 9.7 ± 2.4 | 1.8 ± 0.2 | 10 ± 3 | 26 ± 1 |
| Trp5Leu mutant | ND | 1.9 ± 0.2 | 17 ± 5 | 4.4 ± 0.4 |
| Trp5Val mutant | 7.2 ± 0.5 | 3.0 ± 0.3 | 22 ± 7 | 5.3 ± 0.5 |
| Trp5Ala mutant | ND | 2.0 ± 0.1 | 17 ± 2 | 5.3 ± 0.2 |
ND, not done.
