TABLE 1.
Leukocyte (CD45+) populations isolated from the feet of control mice and the swollen feet of mice infected with the Reunion Island isolate 7 days previouslya
| Cell type | FACS marker(s) | Mean % of cells in control mice ± SE | Mean % of cells 7 days postinfection ± SE |
|---|---|---|---|
| Monocytes | CD11b+ F4/80lo/− | 69 ± 15.5 | 45 ± 9.9 |
| Macrophages | F4/80+ CD11b+ | 22.3 ± 2.6 | 21 ± 4 |
| NK cells | NK1.1+ | 2.3 ± 2 | 16.3 ± 6 |
| CD4 T cells | CD3+ CD4+ | 1.3 ± 2.1 | 5.2 ± 1.3 |
| CD8 T cells | CD3+ CD8+ | 0.2 ± 1.5 | 1.2 ± 0.2 |
| B cells | CD19+ | 3.5 ± 2.5 | 4.6 ± 2 |
| Conventional DC | CD11c+ F4/80− NK1.1− B220− PDCA1− | 0.9 ± 0.1 | 6.4 ± 1.1 |
| Plasmacytoid DC | CD11c+ B220+ PDCA1+ | <0.1 | 3.8 ± 2.1 |
a
Control mice were injected with PBS 7 days previously. Feet from virus-infected animals contained, on average, 31-fold ± 7.7-fold-more CD45+ cells than feet from control animals. Data represent the means of data from two to three experiments with cells from three to five animals pooled. DC, dendritic cells.