FIG. 5.

Northern analysis of ALT RNA in poly(A)-enriched RNA and in multiple cell types. Total or poly(A)-enriched RNA from the indicated cells was subjected to Northern blotting analysis using a radioactively labeled riboprobe that detects ALT RNA. Total RNA or poly(A)-enriched RNA was prepared from BJAB cells, untreated BCBL-1 cells, or BCBL-1 cells treated with valproic acid (VA; 600 μM) for 24 h; untreated BC-3 cells or BC-3 cells treated with valproic acid (600 μM) for 24 h; or SLK cells, untreated iSLK.219 cells, or iSLK.219 cells treated with doxycycline (Dox; 1 μg/ml) for 48 h. Arrows indicate ALT transcript.