FIG. 1.

Schematic overview of the A3H variants used in this study. A3H hapI-GKE and A3H hapII-RDD differ at amino acid positions 105, 121, and 178. The panel of site-directed mutants containing all possible combinations of both haplotypes was used to assess the effect of individual residues on deaminase activity/antiviral activity. The A3H variants used represent the most common splice variant (SV183), are 184 residues long, and contain a FLAG tag at their N terminus (described in reference 20). G, glycine; K, lysine; E, glutamic acid; R, arginine; D, aspartic acid.