FIG. 6.

Gag determinants for A3H packaging into virus-like particles. The different A3H variants (100 ng) and A3G (100 ng) were cotransfected with full-length and progressive Gag deletion expression plasmids (500 ng). Supernatants containing VLPs were clarified and pelleted through a 20% sucrose cushion and analyzed by Western blotting. Anti-FLAG antibodies detected A3H and A3G, while anti-HIV-1 serum was used to detect HIV-1 Gag. Cell lysates were probed in parallel with anti-FLAG and anti-GAPDH. One representative experiment of three independent experiments is shown.