FIG. 4.

RSV-mediated cell-to-cell fusion is inhibited by palivizumab and motavizumab. TREx-F green and TREx-F red were cocultured in equal proportion, followed by induction of F protein expression with tetracycline (tet). The cultures were treated with palivizumab or motavizumab at the time of tetracycline addition. The cultures were analyzed 55 h posttreatment by fluorescence microscopy and flow cytometry. (A) Representative images and corresponding dot plots obtained through flow cytometric analysis are shown. (B) The plots show the average percentage of fused, double-positive cells detected by flow cytometry for each treatment condition. The dashed line indicates the level of background double-positive cells observed in these cultures. The plot represents duplicate data points, along with the SD.