TABLE 1.
CWD infectivity assays
| Dilution | Tg(CerPrP-M132)1536+/− |
Tg(CerPrP-E226)5037+/− |
No. of CPCA units (mean ± SD)a |
|||
|---|---|---|---|---|---|---|
| Incubation time (days) (mean ± SD) | No. of sick mice/total no. of mice | Incubation time (days) (mean ± SD) | No. of sick mice/total no. of mice | Elk pool 1 | Elk pool 2 | |
| 10−2 | 293 ± 31 | 6/6 | 126 ± 9 | 8/8 | 647 ± 184 | 840 ± 189 |
| 10−3 | 233 ± 23 | 6/6 | 128 ± 4 | 7/7 | 196 ± 134 | 341 ± 135 |
| 10−4 | 288 ± 31 | 7/7 | 147 ± 12 | 6/6 | 34 ± 39 | 56 ± 42 |
| 10−5 | 402 ± 3 | 5/5 | 263 ± 79 | 3/6 | 9 ± 9 | 13 ± 25 |
| 10−6 | 436 ± 3 | 2/6 | 248 ± 51 | 3/6 | ND | ND |
| 10−7 | >533 | 0/6 | >537 | 0/6 | ND | ND |
| 10−8 | >543 | 0/6 | >539 | 0/6 | ND | ND |
| 10−9 | >546 | 0/7 | >538 | 0/7 | ND | ND |
| 10−10 | >554 | 0/6 | >553 | 0/6 | ND | ND |
| 10−11 | >566 | 0/8 | >562 | 0/5 | ND | ND |
a
Elk pool 1, elk CWD pool inoculum; elk pool 2, elk CWD pool inoculum passaged in Tg(CerPrP-E226)5037+/− mice; ND, not determined.