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Preferential activation of ERK, but not of p38, Akt, or JNK, in SPL cells. HEK cells or SPL cells were either left uninfected or infected with WSN virus at an MOI of 1. (A) At 1 or 2 dpi, whole proteins were used for Western blot analysis to record the expression of ERK, pERK, STAT1, pSTAT1, viral NP, and actin. (B) Cell lysates were obtained at 1 or 2 dpi and were analyzed by Western blotting to determine the levels of p38, p-p38, Akt, pAkt, JNK, pJNK, and actin.
