TABLE 1.
Primers and probes
| Strain seta | Primer or probe | Sequence (5′-3′) |
|---|---|---|
| C. jejuni/C. coli cadF (set 602) | ||
| Primers | CAMP 221F | CTGCTAAACCATAGAAATAAAATTTCTCAC |
| CAMP 221R | CTTTGAAGGTAATTTAGATATGGATAATCG | |
| Probes | CAMP 221fl | ACATCAGAATAATGCTCTAACCCAAATTCTAAT-FITC |
| CAMP 221fl2 | TCCGAGTAATGTTCTAAACCTAGTTCTAAT-FITC | |
| CAMP 221iLC610 | Red610-CATCACCATCTTCATAGGCTACTTGACCTATAGT-PO4 | |
| Salmonella sp. invA (set 604) | ||
| Primers | SALM 178F | TGCATAATGCCAGACGAAAGAG |
| SALM 178R | ATCATTTCTATGTTCGTCATTCCA | |
| Probes | SALM 178fl | GAGGATTCTGTCAATGTAGAACGACCC-FITC |
| SALM 178iLC610 | Red610-TAAACACCAATATCGCCAGTACGATATTCAGTGCG-PO4 | |
| Shigella sp./enteroinvasive E. coli ipaH (set 663) | ||
| Primers | SHIG 172F | ATAGAAGTCTACCTGGCCT |
| SHIG 172R | GGGAGAACCAGTCCGTAA | |
| Probes | SHIG 172fl | CAAATGACCTCCGCACTGCC-FITC |
| SHIG 172iLC670 | Red610-AGCCACGGTCAGAAGCCG-PO4 | |
| Yersinia sp. lysP (set 664) | ||
| Primers | lysP 156F | GGCATCATGAAAGGCGG |
| lysP 156R | TGATTCACCAGCAGCAATAC | |
| Probes | lysP 156fl | GGTTCTCGGCGATGATTGGTGTGG-FITC |
| lysP 156iLC670 | Red610-ATGATTGTCGGTTTCTCCTTCCAGGGAACTGAGC-PO4 |
a
Sets: TIB MolBio, Adelphia, NJ.