TABLE 3.
MTBDRsl assay and sequencing results for gyrA mutation of 42 phenotypically FQ-resistant strains included in the study
| Strain | MTBDRsl assay results |
GyrA sequencing result (mutation detected)c | Concordanced | ||
|---|---|---|---|---|---|
| FQ resulta | Hybridization patternb | Mutation detected | |||
| FQ1091 | R | WT + MUT3C | D94G | D94G* | Yes |
| FQ1092 | R | ΔWT3 + MUT3A | D94A | D94A | Yes |
| FQ1093 | R | ΔWT3 + MUT3C | D94G | D94G | Yes |
| FQ1094 | R | ΔWT3 + MUT3C | D94G | D94G | Yes |
| FQ1095 | R | WT + MUT3A | D94A | D94A* | Yes |
| FQ1150 | R | WT + MUT3C | D94G | D94N | No |
| FQ1151 | R | ΔWT3 + MUT3C | D94G | D94G | Yes |
| FQ1152 | R | WT + MUT3C | D94G | D94G* | Yes |
| FQ1153 | R | ΔWT2 + MUT1 | A90V | A90V | Yes |
| FQ1154 | S | WT | None (WT) | None | Yes |
| FQ1155 | R | ΔWT3 | D94X | D94Y | Partial |
| FQ1156 | R | ΔWT2 + MUT1 | A90V | A90V | Yes |
| FQ1157 | S | WT | None (WT) | None | Yes |
| FQ1158 | R | ΔWT2 + MUT1 | A90V | A90V | Yes |
| FQ1159 | R | ΔWT3 + MUT3A | D94A | D94A | Yes |
| FQ1160 | S | WT | None (WT) | None | Yes |
| FQ1161 | S | WT | None (WT) | None | Yes |
| FQ1162 | R | ΔWT3 + MUT3C | D94G | A90V*/D94G* | Partial |
| FQ1281 | R | WT + MUT3C | D94G | A90V*/D94G* | Partial |
| FQ1283 | R | WT + MUT1 + MUT3B + MUT3C | A90V/D94N/D84Y and D94G | A90V*/D94G* | Partial |
| FQ1284 | S | WT | None (WT) | None | Yes |
| FQ1286 | R | ΔWT3 + MUT3C | D94G | D94G | Yes |
| FQ1288 | S | WT | None (WT) | None | Yes |
| FQ1289 | R | WT+ MUT1 + MUT3A | A90V/D94A | A90V*/D94A* | Yes |
| FQ1290 | R | ΔWT3 + MUT3A | D94A | D94A | Yes |
| FQ1291 | R | ΔWT3 + MUT3C | D94G | D94G | Yes |
| FQ1292 | R | ΔWT3 + MUT3A + MUT3C | D94A/D94G | D94G*/D94A* | Yes |
| FQ1293 | R | ΔWT3 + MUT3A | D94A | D94A | Yes |
| FQ1294 | S | WT | None (WT) | None | Yes |
| FQ1295 | R | WT + MUT3C | D94G | D94G* | Yes |
| FQ1296 | R | ΔWT3 + MUT3C | D94G | D94G | Yes |
| FQ1297 | R | ΔWT3 + MUT3B + MUT3C | D94N/D94Y and D94G | D94G* | Partial |
| FQ1298 | R | ΔWT3 + MUT3B + MUT3C | D94N/D94Y and D94G | D94G* | Partial |
| FQ1318 | S | WT | None (WT) | None | Yes |
| FQ1319 | R | WT + MUT3B | D94N/D94Y | None | No |
| FQ1320 | R | ΔWT2 + MUT1 | A90V | A90V | Yes |
| FQ1321 | R | ΔWT2 + MUT1 | A90V | A90V | Yes |
| FQ1322 | R | ΔWT3 + MUT3C | D94G | D94G | Yes |
| FQ1323 | R | ΔWT2 + MUT1 | A90V | A90V | Yes |
| FQ1324 | S | WT | None (WT) | None | Yes |
| FQ1325 | S | WT | None (WT) | None | Yes |
a
Abbreviations: R, resistant; S, susceptible (result for FQ DST by MTBDRsl assay).
b
The hybridization pattern shows the probes the sample hybridized to. WT, wild type; ΔWT indicates lack of hybridization to the wild-type probe.
c
Asterisks indicate that the mutation was found in different peaks.
d
Partial concordance means that at least one identical mutation was detected by both tests.