FIG. 2.
Amplification of known parasite equivalents of the Padley international standard with the PfMt19 primer. The figure shows the increase in turbidity with time in reaction wells containing 20,000, 2,000, 200, 20, or 2 parasite genome equivalents of purified DNA. The ISPf was diluted in blood to a given concentration (assuming an initial parasite density of 5 × 105 parasites per μl in the ISPf starting material) and then extracted using QiaAMP blood mini-kits. Reactions were performed using an LA-320CE turbidimeter. SDW, sterile distilled water.