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Pulsed-field gel electrophoresis. Genomic DNA was digested with BssHII enzyme (8 U; New England Biolabs, Beverly, MA) overnight at 37°C. Samples were run in a 1% agarose gel with a switch time of 2.2 to 17.6 s and a field strength of 6 V/cm for 23 h on a contour-clamped homogeneous electric field (CHEF) apparatus (Bio-Rad). Lane A, C. jejuni reference strain 81-176; lane B, inoculum strain C. jejuni CG8421; lane C, macrolide-resistant C. jejuni CG8421 isolate from day 21.
