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a Bead fluorescence versus biotin-FITC concentration from on-chip incubation and detection using a single-stage incubation device. b Mean fluorescence intensity versus biotin-FITC concentration response curve from microdevice detection following benchtop incubation for 30 s to study streptavidin–biotin binding over an extended concentration range. Bead fluorescence saturation is evident at biotin-FITC concentrations higher than 300 ng/ml
