Table 1.
ELISA sensitivity, specificity and interferon-γ recall responses in C57BL/6 and HLA-DR4 transgenic mice using Coxiella burnetii recombinant proteins
| ID | Molecular mass (kDa) |
Predictive function | Specificity (%) |
Sensitivity (%) |
Presented by murine MHC II |
Presented by HLA DRA |
|---|---|---|---|---|---|---|
| NMI BBS | 87.5 | 85 | √ | √ | ||
| CBU0383 | 24 | DNA-3-methyladenine glycosidase I | 87.5 | 31.6 | √ | √ |
| CBU0612 | 19 | Outer membrane protein OmpH, putative | 81.2 | 51.6 | √ | |
| CBU0718 | 10.4 | Hypothetical membrane-associated protein | 78.1 | 45 | √ | |
| CBU0781 | 38.5 | Ankyrin repeat domain protein | 81.3 | 40 | √ | √ |
| CBU0891 | 34.4 | Hypothetical exported membrane protein | 80.6 | 41.6 | √ | |
| CBU0311 | 29 | Major outer membrane protein porin (P1) | 78.1 | 43.3 | √ | √ |
| CBU0395 | 25 | 5 Lipoprotein, putative | 90 | 28.3 | √ | |
| CBU0952 | 28 | Outer membrane protein AdaA | 100 | 25 | ||
| CBU1221 | 22 | Lipoprotein, putative | 81.2 | 48.3 | √ | |
| CBU1716 | 35 | Glycine cleavage system T-protein | 90 | 46.6 | √ | √ |
| CBU1910 | 27 | Outer membrane protein (Com1) | 90 | 50 | √ | √ |
HLA, human leukocyte antigen; MHC, major histocompatibility complex.
Sensitivity was calculated using number of ELISA-positive samples from the IFA-positive pool divided by the total number of IFA-positive samples. Specificity was calculated using the number of ELISA-negative samples from the IFA-negative pool divided by the total number of IFA-negative samples. A tick mark (√) indicates a stimulation index higher than three (stimulation index was calculated using number of spot-forming cells in vaccinated mice divided by number of spot-forming cells in naïve mice). NMI BBS was prepared from a solubilized fraction of mechanically lysed Nine Mile phase I (RSA493).