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. Author manuscript; available in PMC: 2011 Apr 22.
Published in final edited form as: J Med Chem. 2010 Apr 22;53(8):3320–3329. doi: 10.1021/jm100047k

Figure 5. Western Immunoblot Analysis of of ERα Protein Level and Subcellular Distribution with Compound and Estradiol Treatment.

Figure 5

MCF-7 cells were treated with 1 μM compound for 16h and then ±1 nM estradiol for 4h prior to cell fractionation and monitoring of ERα by Western immunoblots; leftmost two lanes show vehicle or E2 treatment for 20 h. Sp1 was monitored as an internal reference for gel loading.