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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: FEBS J. 2010 Jun;277(11):2550–2553. doi: 10.1111/j.1742-4658.2010.07670.x

Figure 1. Displacement of [γ32P]-8-azido-ATP by staurosporine.

Figure 1

(A) Qalitative analysis. The ANF-RGC ARM domain protein (1 μg of protein for one reaction) was UV cross-linked with [γ32P]-8-azido-ATP in the absence or presence of 100 μM staurosporine or 1 mM ATP. Lane “0” - only [γ32P]-8-azido-ATP present; lane “1 mM ATP” – 1 mM ATP was present in addition to [γ32P]-8-azido-ATP; lane “100 μM S” – 100 μM staurosporine was present in addition to [γ32P]-8-azido-ATP. The reaction mixtures were analyzed through SDS-15%PAGE and autoradiographed. The radioactive band corresponding to the [γ32P]-8-azido-ATP cross-linked ARM domain is indicated on both sides of the autoradiogram by arrows. (B) Quantitative analysis. The ANF-RGC ARM domain protein (1 μg of protein for one reaction) was UV cross-linked with [γ32P]-8-azido- ATP in the presence of indicated concentrations of staurosporine (closed circles) or ATP (open circles). Radiolabeled bands were cut out from the gel, counted for radioactivity and the percentage of radioactivity retained was calculated. The experiment was repeated three times and the results presented are mean ± SD of these experiments.