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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: FEBS J. 2010 Jun;277(11):2550–2553. doi: 10.1111/j.1742-4658.2010.07670.x

Figure 5. Staurosporine mimics the allosteric ATP effect on ANF-dependent ANF-RGC activity.

Figure 5

(A) Membranes of COS cells expressing ANF-RGC were incubated with 10−7 M ANF in the presence of indicated concentrations of staurosporine, AMP-PNP or ATP. (B) Membranes of COS cells expressing ANF-RGC were incubated with 10−7 M ANF and/or 0.5 mM AMP-PNP, 10 μM staurosporine or were incubated with 10−7 M ANF, 0.5 mM AMP-PNP and 10 μM staurosporine. The experiments were done in triplicate and repeated three times. The values presented are average (± SD) from these experiments.