Figure 7. The age3Δ mutant strain is susceptible to several unrelated metabolic inhibitors.

The strains SN87 (AGE3+/+), UZ22 (AGE3+/Δ), UZ45 (age3Δ/Δ) and UZ55 (age3Δ/Δ::AGE3) were grown in YPD medium at 30°C to an OD₆₀₀ of 0.5. After washing, the cells were spotted in serial dilutions (cell number per spot is given) onto agar plates containing distinct concentrations of the toxic compounds mentioned and grown for two or three days at 30°C. The homozygous age3 mutant cells are susceptible to the azole drugs miconazole and itraconazole, hygromycin B (HygB), cadmium ions, SDS and brefeldin A, but not to the antifungal drugs amphotericin B (AmB) and 5-fluorocytosine (5-FC). Note, that as observed for other phenotypes, the heterozygous and the reintegrant strain show an intermediate susceptibility between the wild-type and the homozygous mutant strains. In the experiments shown in the upper panel two independent clones (1 and 2) of the construction of the homozygous age3Δ strain were spotted. Since these clones behaved identical, only clone 1 was used in later experiments (lower panel).