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. 2010 Aug 5;6(8):e1001052. doi: 10.1371/journal.pgen.1001052

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Coelho et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) T1 to T8 refer to 8 individually germinated teliospores obtained by micromanipulation from the cross between strains CBS 6832 (A2–15) and ML 2241 (A1–3). The result of the diagnostic PCRs for the presence of the alternate STE3.A1 and STE3.A2 genes and the identification of HD1/HD2 amplicons by Rsa I digestion is shown. Screening of the parental origin of the DMC1 allele by PCR and digestion with Msp I show clonality of the various colonies examined for each teliospore. For each germinated teliospore, three to six colonies were studied. With the exception of teliospore T1, only one mating type was recovered per meiosis presumably due to asynchronous germination of basidiospores (see Figure S5C). Teliospore T8 yielded a strain which is apparently diploid, and the new mating type was recovered from teliospore T7.