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. Author manuscript; available in PMC: 2011 Aug 6.
Published in final edited form as: Cell Stem Cell. 2010 Aug 6;7(2):240–248. doi: 10.1016/j.stem.2010.06.017

Figure 2. Knockdown of Non-Muscle Myosin Heavy Chains (MYH) Reduces Blebbing and Improves Survival and Cloning Efficiency of Human ES Cells.

Figure 2

Knockdown (by siRNA) of MYH9 alone or in combination with MYH10:

(A) changed ES cell morphology in colonies 96 hours after transfection,

(B) reduced blebbing immediately after dissociation,

(C) increased attachment/spreading 5 hr after plating,

(D) increased (*p<0.05) survival 24 hr after plating, and

(E) increased (*p<0.05) cloning efficiency. Comparable results were obtained in multiple experimental repetitions using both H1 (n=15) and H9 human ES cell lines (n=2).