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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Pacing Clin Electrophysiol. 2009 Dec 16;33(3):274–285. doi: 10.1111/j.1540-8159.2009.02642.x

Figure 5. Rescue of SCN5A-P1008S channels.

Figure 5

A: Representative sodium current traces recorded from WT, P1008S, P1008S incubated with mexiletine (300 µM) treatment and P1008S incubated at 22°C. All were recorded at 48 hours after transfection. Mexiletine was washed out 30 minutes prior to recording the traces. INa was elicited by depolarizing pulses ranging from −90 mV to +30 mV in 5 mV increments (holding potential=−120 mV). The inset on top depicts the voltage-clamp protocol employed. B: Current density-voltage relationship for WT (open circles, n = 33), P1008S (filled circles, n=15), P1008S incubated with 300 µM mexiletine (24 hours, open down-triangles, n=10; 48 hours, filled down-triangles, n=9) and P1008S incubated at 22°C (24 hours, open up-triangles, n = 8; 48 hours, filled up-triangles, n=11). C: Summary of INa amplitudes in WT, and in P1008S under control and after 24 or 48 hrs incubation with either 300 µM mexiletine or room temperature. All incubations were done at 37°C except for the room temperature group. (**P<0.01 and #,*P<0.05).