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. 2010 Aug 5;6(8):e1001035. doi: 10.1371/journal.ppat.1001035

Figure 1. b-dependent gene expression.

Figure 1

In U. maydis strains AB31 and AB33, expression of the bE1 and bW2 genes is controlled via the arabinose-inducible crg1-promoter and the nitrate-inducible nar1-promoter, respectively. Strains AB32 and AB34 are isogenic to AB31 and AB33, respectively, but harbor the incompatible bE2 and bW2 genes as control. bE/bW genes were induced by shifting the cells to array medium containing arabinose (AB31 and AB32) or nitrate (AB33 and AB34). Cells were harvested at the given time points, RNA was extracted and used for microarry-analysis (Affymetrix MPIUstilagoA gene chip). The heat-map indicates the significant changes (adjusted P<0.01; change >2) in gene expression for AB31 and AB33 at a given time point relative to the control strains AB32 and AB34. Order of genes from top to bottom and expression changes are given in Suppl. Table S1. Details for the statistics and filters applied are given in “Material and Methods”.