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. 2010 Aug 5;6(8):e1001037. doi: 10.1371/journal.ppat.1001037

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Nilsson et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Display of the splicing profile for Tb927.6.4240 using Gbrowse. Spliced leader tags from bloodstream form (red) and procyclic form (green) are shown. Numbers indicate tags per million. The position and size of the RT qPCR amplicon is depicted by a black bar (RT qPCR amplicon). Below the bar the ratios of mRNA abundance as determined by SLT and qPCR respectively are shown. (B) Expected size of PCR products using a spliced leader-specific oligonucleotide together with a gene specific oligonucleotide are depicted. Gel shows PCR products from procyclic (PC) and bloodstream (BS, long slender) cDNA.