Table 1. Library statistics.

	Long slender bloodstream form	Short stumpy bloodstream form	Procyclic form
Number of reads	2,223,504	1,919,710	1,430,649
Number of reads ≥24nt	1,814,248	1,602,918	1,171,012
Number of reads aligned1	1,562,364	1,538,057	1,148,155
Number of splice sites	29,406	27,572	23,842
Genes with tags	8,277	8,173	7,926
Coverage in %2	91	90	87
Genes with major internal sites	793	870	683
Genes with major internal sites but no downstream AUG	97	95	90
Genes with only internal sites	542	558	496
Genes with 5′ UTR >2kb	198	191	153
Genes with major 5′ UTR >2kb	356	329	235
Genes with major 5′ UTR <2kb	7,143	6,989	7,009
Genes with ≥1 uORF	1,331	1,326	1,229
Splice sites/gene mean	2.9	2.8	2.7
Splice sites/gene median	2	2	2
5′ UTR length mean (0–2kb)	139	127	105
5′ UTR length median (0–2kb)	47	32	34
Tags per gene mean	85.1	94.8	96.9
Tags per gene median	12	15	24

¹reads aligned to one of the 11 megabase-sized chromosomes excluding expression sites or VSGs.

²of 9068 genes in the genome.