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. 2010 Jul 20;10:194. doi: 10.1186/1471-2180-10-194

Figure 1.

Figure 1

Southern blot hybridization of EcoRI-restricted M. suis DNA showing the genomic location of the ms262 clone insert on a 1.2- and a 2.7-kb fragment. (A) agarose gel electrophoresis of EcoRI-restricted DNA. (B) the blot probed with the DIG-labeled 950 bp-EcoRI fragment of the library clone ms262; (C) the blot probed with the DIG-labeled 1050 bp-EcoRI fragment of the library clone ms262; (M) molecular weight standard; (Ms) M. suis. The arrows indicate the positions of the hybridized 1.2- and 2.7-kb fragments. (D) schematic map of the ORF localisation on the library clone ms262. The grey box arrows indicate the two ORFs: ppa (inorganic pyrophosphatase) and trx (thioredoxin).