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. 2010 Jul 20;10:194. doi: 10.1186/1471-2180-10-194

Figure 3.

Figure 3

Expression and immunological characterization of the M. suis sPPase. (A) Coomassie-stained SDS polyacrylamide gel electrophoresis of recombinant M. suis PPase., Co, non-induced IMAC purified E. coli lysate; PPA, IMAC purified recombinant PPase. (B) Immunoblot analysis of recombinant PPase and M. suis whole cell antigen; immunological detection with anti-PPase rabbit immune serum; M, molecular weight standard; PPA, recombinant PPase; Ms, purified M. suis cells; Co, non-induced IMAC purified E. coli lysate. (C) Immunoblot of recombinant PPAse; immunological detection with a serum pool from experimentally infected pigs; PPA, recombinant PPase; Co, non-induced IMAC purified E. coli lysate.