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. 2010 Jul 22;10:196. doi: 10.1186/1471-2180-10-196

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Copyright ©2010 Foy et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Response of the B. cereus lysK T-box regulatory element to reduced levels of charged tRNA^Asn. A) The mixed codon box for lysine and asparagine. (B) Growth (open symbols) and β-galactosidase activity (closed symbols) of NF60 (P_spacasnS P_{lysK Tbox}lacZ pMAP65) in LB containing 1 mM (diamonds) and 600 μM (triangles) IPTG. (C) Northern analysis of tRNA^Lyscharging in wild-type B. subtilis strain 168 and strain NF60 growing in LB media with the indicated IPTG concentrations. The percentage of charged tRNA^Lysis indicated beneath each lane. The profiles presented are representative.