Table 2.
Bacterial strains and plasmids used in this work
| Strains, plasmids | Relevant characteristics | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| TG-1 | supE hsdΔ5 thi Δ(lac-proAB) F'[traD36] | [24] |
| TP611 | recBC hsdR-M- cyab10pcn | [25] |
| B. cereus 14579 | wild type isolate | [34] |
| B. subtilis | ||
| 168 | trpC2 | Laboratory stock |
| 1A717 | amyE::erm EmR | [32] |
| 1A765 | trpC2 lys | [35] |
| NF33 | trpC2 lys amyE::pBCJ307 (PlysK (T box) lacZ) CmR | This study |
| NF52 | trpC2 amyE::pNF48 (PlysK (T box) lysK) SpecR | This study |
| NF54 | trpC2 amyE::pNF48 (PlysK (T box) lysK) SpecR DlysS KanR | This study |
| NF58 | trpC2 amyE::pBCJ307 (PlysK (T box) lacZ) CmR asnS::pNF40 (Pspac asnS) EmR | This study |
| NF60 | trpC2 amyE::pBCJ307 (PlysK (T box) lacZ) CmR asnS::pNF40 (Pspac asnS) EmRpMap65 (Ppe -lacI) PhR | This study |
| NF113 | trpC2 lysS::pNF112 (PlysK (T box) lysS) CmR | This study |
| NF204 | trpC2 amyE::pBCJ307 (PlysK (T box) lacZ) EmR CmR | This study |
| NF205 | trpC2 lysS::pNF112 (PlysK (T box) lysS) CmR amyE::pBCJ307(PlysK (T box) lacZ) EmR | This study |
| NF206 | trpC2 amyE::pNF48 (PlysK (T box) lysK) SpecR DlysS:KanR amyE::pBCJ307(PlysK (T box) lacZ)CmR | This study |
| BCJ363 | trpC2 amyE::pBCJ307 (PlysK (T box) lacZ) CmR | This study |
| BCJ366 | trpC2 amyE::pBCJ307 (PlysK (T box) lacZ) CmR lys::pXZ2 (Pspac lysS) EmR | This study |
| BCJ367 | trpC2 amyE::pBCJ307 (PlysK (T box) lacZ) CmR lys::pXZ2 (Pspac-lysS) EmR pMap65 PhlR | This study |
| Plasmids | ||
| pBluescript2 KS(-) | cloning vector ApR | Stratagene, La Jolla CA |
| pMutin4 | integration vector for B. subtilis | [27] |
| pBCJ102 | pBluescript based vector containing transcription terminator cassettes ApR | [29] |
| pBCJ144 | vector to replace part of B. subtilis lysS with KanR | [29] |
| pBCJ307 | vector with transcriptional fusion of B. cereus lysK promoter and T box with lacZ CmR | This study |
| pDG268 | vector to generate lacZ promoter fusions at the amyE locus by double crossover ApR CmR | [26] |
| pDG1730 | vector for integration at the amyE locus in B. subtilis SpecR | [30] |
| pXZ2 | Vector to placing B. subtilis lysS under control of IPTG inducible Pspac promoter EmR | This study |
| pMUTIN-XZ | pMUTIN4 with the lacZ gene removed EmR | This study |
| pMap65 | replicating B. subtilis plasmid encoding penP-lacI PhlR KanR | [28] |
| pNF30 | plasmid with B. cereus lysK promoter and T box element in pBCJ102 ApR | This study |
| pNF40 | B. subtilis asnS promoter on 516 bp fragment in pMUTIN-XZ | This study |
| pNF48 | B. cereus lysK promoter and lysK gene cloned into pDG1730 | This study |
| pNF112 | the lysK promoter and T box element (423 bp) fused to B. subtilis lysS (672 bp fragment) | This study |