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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Insect Biochem Mol Biol. 2010 Apr 10;40(6):429–439. doi: 10.1016/j.ibmb.2010.04.002

Fig. 6.

Fig. 6

Ultrathin longitudinal section of the ovariole showing oocyte development at different stages in the germarium of malE RNAi (control, A & B) and EcR RNAi (C & D) on day 4 PAE. Panel A, Neck region showing germarium with developing secondary oocyte surrounded by follicular cells; Tropharium with nurse cells occupy the distal portion of the germarium; black circle- boundary of the secondary oocyte; White circle- boundary of the surrounding follicle cells. Panel B, Enlarged circled area of A with the follicle cells surrounding the secondary oocyte is numbered 1–10. Panel C, Germarium and tropharium regions of EcR RNAi beetle ovariole; Black circle, boundary of the primary oocyte; White circle- boundary of the surrounding follicle cells. Panel D, Germarium region circled in C at higher magnification; the primary oocyte is surrounded by follicular cells; also seen are trophic cards and nurse cells. GM, Germarium; NC, Nurse cells; NCn, Nurse cell nucleus; Oo, Oocyte; Oon, Oocyte nucleus; PFC, follicular cell; TC, Trophic card; TM, Tropharium. All micrographs are arranged with tropharium on the top and germarium towards the bottom. N=4. Scale Bar: A,C − 5 µm; B, D − 2 µm.