Table 1.
Gene expression in the ovary Vs whole body of female beetles on day 4 PAE by microarray analysis
| Gene ID | Ovary 4 day PAE |
Whole body 4 day PAE |
Fold change |
p-value | Annotation |
|---|---|---|---|---|---|
| Vitelligenin receptors | |||||
| TC004042 | 5.525579 | 0.846001 | 6.53141 | 0.000237 | VgR1 |
| TC008425 | 4.12203 | 1.03703 | 3.97483 | 0.000683 | VgR2 |
| 20E biosynthesis and action | |||||
| TC011231 | 4.054114 | 1.131469 | 3.583052 | 1.77E-05 | Shade |
| TC014274 | 3.762641 | 1.076641 | 3.494796 | 3.86E-05 | Shadow |
| TC001916 | 3.180004 | 0.991075 | 3.208642 | 0.000386 | Phantom |
| TC015030 | 3.52548 | 1.102684 | 3.197181 | 1.82E-05 | Spookier- related |
| TC004159 | 4.223212 | 1.368588 | 3.085817 | 0.00384 | Spookier |
| TC014691 | 2.358138 | 0.874333 | 2.697071 | 0.000155 | Disembodied |
| TC012113 | 2.530572 | 0.964173 | 2.624605 | 0.000295 | EcR |
| TC003935 | 2.28661 | 1.131274 | 2.02127 | 0.00278 | E78 |
| TC004598 | 2.576739 | 0.920134 | 2.800394 | 0.000224 | HR78 |
| JH metabolism | |||||
| TC003437 | 5.70203 | 0.70476 | 8.090737 | 0.00302 | JHEH |
| TC002992 | 2.627899 | 0.833283 | 3.153668 | 0.000894 | JHEH |
| TC013193 | 2.09687 | 0.75478 | 2.778123 | 3.82E-05 | JHE |
The total RNA isolated from the whole body and ovary of the female beetle at 4 day post adult emergence (PAE) were used for microarray analysis. The data filtered using Benjamini-Hochberg false discovery rate multiple testing corrections generated by GeneSpring GX v.9.0.1 software were used. The normalized expression levels of selective genes involved in vitellogenesis, JH and 20E pathways at different fold changes (whole body Vs ovary) with p-value of less than 0.05 for each gene are shown for comparison.