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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Comp Biochem Physiol B Biochem Mol Biol. 2010 Apr 22;156(4):298–308. doi: 10.1016/j.cbpb.2010.04.008

Figure 4. Leu2 selection measured by dilution plating in the yeast-two-hybrid assay indicates interaction of M. trossulus MDM with p53 in galactose-based, leu- medium.

Figure 4

A) Mdm background: Yeast cells transfected with LexA-Mdm fusion plasmid, B) P53 background: Yeast cells transfected with B42-p53 fusion plasmid, C) MDM and p53: Mated yeast containing both plasmids. Symbols: ◇, galactose-based media containing leucine (Gal + Leu); □, galactose-based media, no leucine (Gal −Leu); and △, glucose-based media, no leucine (Glu −Leu).