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. Author manuscript; available in PMC: 2011 Aug 15.
Published in final edited form as: J Immunol. 2010 Jul 21;185(4):2324–2330. doi: 10.4049/jimmunol.0903052

Figure 2. ER stress increases transcription factor and machinery occupancy of the ifnb1 promoter.

Figure 2

RAW 264.7 macrophages were pre-treated with Tpg for 1h and then stimulated with LPS for the times indicated. Binding of IRF-3, CBP, TBP and Pol II to the ifnb1 promoter was detected by ChIP. Relative factor occupancy compared to input chromatin was determined by qPCR. Control IgG results were combined for all stimulation conditions (IgG, no symbol). Results were combined from 4 (IRF-3, TBP), 5 (CBP), and 2 (Pol II) independent experiments. *P<0.001 for LPS vs. Tpg+LPS.