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. Author manuscript; available in PMC: 2011 Aug 15.
Published in final edited form as: J Immunol. 2010 Jul 21;185(4):2324–2330. doi: 10.4049/jimmunol.0903052

Figure 3. XBP-1 knockdown decreases synergistic induction of IFN-β in macrophages.

Figure 3

A) RAW 264.7 macrophages transfected with 300 nM control or XBP-1 siRNA (XBP-1i) were treated with 1h Tpg and then 3h LPS. XBP-1 (top) or actin (bottom) was detected by Western. Results are representative of 2 separate experiments. B) RAW cells transfected with 300 nM control RNAi or XBP-1i were stimulated as in (A) and relative IFN-β (top) and ERdj4 (bottom) mRNA was determined by qPCR. Results were combined from 2 (ERdj4) and 3 (IFN-β) independent experiments. *P=0.029, **p=0.021. C) RAW cells transfected with 200 nM control RNAi (black) or XBP-1i (gray) were stimulated as in (A) and relative expression of IFN-β, 18S rRNA, and IL-1β, and IL-6 mRNA was determined by qPCR. Results are representative of 3 independent experiments.