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. 2010 May 24;588(Pt 14):2657–2668. doi: 10.1113/jphysiol.2010.191395

Figure 4. Stable biochemical interaction between MiRP3 and Kv4.2.

Figure 4

COS-7 cells transfected with MiRP3 (a), MiRP3 and Kv4.2 (b), MiRP3 and Kv4.2–1d4 (c), or Kv4.2–1d4 alone (d), were subjected to lysis and immunopurification (IP) with anti-1d4 antibodies. An additional treatment group (e) consisted of lysate from cells expressing MiRP3 mixed with lysate from cells expressing Kv4.2–1d4, at 4°C for 16 h prior to immunopurification. Blots were probed with antibodies to Kv4.2 (70 kDa) and MiRP3 (21–25 kDa).