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. 2010 May 24;588(Pt 14):2657–2668. doi: 10.1113/jphysiol.2010.191395

Figure 5. Ternary interactions between MiRP3, Kv4.2 and KChIP2.

Figure 5

A, MiRP3 was immunopurified from lysates of tsA201 cells transfected with MiRP3 (a), MiRP3, Kv4.2 and KChIP2 (b), MiRP3 and KChIP2 (c), or Kv4.2 and KChIP2 (d). B, KChIP2 was immunoprecipitated from lysates of tsA201 cells transfected with KChIP2 (a), MiRP3 and KChIP2 (b), MiRP3, Kv4.2 and KChIP2 (c), Kv4.2 and KChIP2 (d), or MiRP3 and Kv4.2 (e). Images of blots span roughly 65–80 kDa for Kv4.2, 24–31 kDa for KChIP, and 20–28 kDa for MiRP3. Faint bands at 25–26 kDa in all five lanes of the IP blotted for MiRP3 are presumed to be crossreactivity of secondary antibodies to immunoprecipitated KChIP2 antibody.