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. 2010 May 24;588(Pt 14):2657–2668. doi: 10.1113/jphysiol.2010.191395

Figure 6. Interactions between filamin A, MiRP3 and Kv4.2.

Figure 6

A, COS-7 cells were transfected with fil–1d4 (a), MiRP3 and Kv4.2 (b), MiRP3, fil–1d4 and Kv4.2 (c), MiRP3 and fil–1d4 (d), or fil–1d4 and Kv4.2 (e). Cells were lysed and immunopurified (IP) with anti-1d4 antibodies. Note that low quantities of co-purified Kv4.2 required high-sensitivity scanning to detect signal. B, co-purification of MiRP3 with Kv4.2 in filamin-replete (A7) and filamin-deplete (M2) cells. Western blots show lysates and epitope-purified fractions from cells transfected with MiRP3 (a), Kv4.2–1d4 (b), or MiRP3 and Kv4.2–1d4 (c).