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. 2010 Jun 21;12(3):R38. doi: 10.1186/bcr2592

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Copyright ©2010 Oloumi et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Overexpression of the human ILK gene. (a) Mouse MMTV-Wnt1 and human MMTV-ILK transgene constructs (b). Quantitative RT-PCR performed on randomly selected tumors from each MMTV-Wnt1 and MMTV-Wnt/ILK group (n = 8) showing a dramatic overexpression of the human ILK gene in the double-transgenic samples. The bar graphs show the relative amount of human ILK overexpression in the individual MMTV-Wnt/ILK and MMTV-Wnt1 tumors normalized for β-actin expression. The statistical analysis with 95% confidence interval of (+754.18; -256.46) shows an average value of about 400-fold of overexpression in human ILK in MMTV-Wnt/ILK with an adjusted P value of 3.1E-16 determined by Benjamini-Hochberg test.