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. Author manuscript; available in PMC: 2010 Aug 5.
Published in final edited form as: Mol Endocrinol. 2007 May 15;21(8):1861–1876. doi: 10.1210/me.2006-0101

Fig. 2. Inhibition of HER4 tyrosine phosphorylation blocks heregulin-induced growth inhibition.

Fig. 2

A, SUM44 cells were cultured for 6 days, treated with different doses of GW572016 for 1 h, and then with or without heregulin (10 ng/ml) for 15 minutes. The cells were lysed and the lysates were immunoprecipated with anti-HER2, anti-HER3, or anti-HER4 antibody and blotted with anti-phosphotyrosine antibody. B, SUM44 cells were grown with or without heregulin (10 ng/ml) in the presence or absence of GW572016 (1 μM) for 6 days, at which time the number of cells were counted. Mean ± SD of triplicates are shown, representative of 3 experiments. ***, P < 0.001 versus control.