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. Author manuscript; available in PMC: 2010 Aug 5.
Published in final edited form as: Curr Protoc Protein Sci. 2001 May;CHAPTER:Unit–11.10. doi: 10.1002/0471140864.ps1110s08

Table 11.10.7.

PTH Amino Acid Yields (Uncorrected) from 15-Cycle Sequence Using 10 pmol β-Lactoglobulina

A D E F G H I K L M N P Q R S T V W Y
1 1.81 0.45 0.79 0.45 2.41 0.36 0.80 1.95 [6.86] 0.32 0.48 0.41 1.03 0.48 3.21d 0.93 0.83 0.10 0.65
2 0.92 0.47 0.60 0.34 1.91 0.15 [7.76] 0.86 0.75 0.15 0.45 0.48 0.59 0.62 1.73 0.45 0.46 0.00 0.44
3 0.70 1.20b 0.57 0.28 1.54 0.16 0.76 0.58 0.66 0.13 0.42 0.60 0.51 0.47 0.94 0.37 [5.95] 0.00 0.42
4 0.66 0.78 0.59 0.24 1.52 0.20 0.53 0.53 0.67 0.13 0.28 0.54 0.61 0.66 0.79 [4.00] 0.60 0.13 0.43
5 0.76 0.98 1.15c 0.25 1.49 0.16 0.51 0.52 0.83 0.10 0.38 0.56 [5.24] 0.60 0.67 0.48 0.47 0.00 0.42
6 0.78 0.56 0.71 0.17 1.48 0.11 0.41 0.42 1.03 0.13 0.38 0.60 0.91 0.72 0.65 [3.47] 0.58 0.00 0.50
7 0.74 0.88 0.76 0.30 1.53 0.19 0.44 0.43 1.02 [4.36] 0.38 0.56 0.64 0.68 0.64 0.53 0.55 0.00 0.50
8 0.77 0.63 0.80 0.26 1.68 0.16 0.30 [3.59] 0.98 0.51 0.38 0.49 0.66 0.66 0.72 0.40 0.52 0.09 0.52
9 0.74 0.66 0.76 0.34 [5.05] 0.14 0.60 0.90 1.02 0.23 0.37 0.47 0.69 0.70 0.67 0.35 0.52 0.03 0.55
10 0.84 0.62 0.75 0.36 2.05 0.09 0.53 0.55 [4.99] 0.20 0.35 0.46 0.69 0.80 0.72 0.32 0.60 0.04 0.56
11 0.87 [3.66] 0.75 0.37 1.83 0.09 0.00b 0.51 1.61 0.26 0.35 0.45 0.68 0.69 0.78 0.27 0.61 0.00 0.59
12 0.88 0.99 0.76 0.37 1.83 0.13 [4.85] 0.56 1.29 0.26 0.26 0.42 0.67 0.73 0.77 0.27 0.62 0.00 0.54
13 0.85 0.72 1.16c 0.35 1.87 0.14 1.10 0.49 1.26 0.24 0.37 0.45 [4.07] 0.68 0.73 0.26 0.58 0.05 0.62
14 0.87 0.67 0.83 0.42 1.89 0.14 0.74 [2.97] 1.21 0.23 0.39 0.22 1.15 0.79 0.81 0.30 0.57 0.03 0.58
15 0.90 0.64 0.76 0.41 1.92 0.08 0.67 0.94 1.27 0.23 0.42 0.51 0.80 0.79 0.79 0.32 [3.71] 0.04 0.57
a

The assigned sequence is indicated by brackets.

b

Value incorrect due to incorrect baseline.

c

Glu value has increased due to partial deamidation of Gln in cycle.

d

Ser contamination is common in cycle 1.