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. Author manuscript; available in PMC: 2010 Aug 6.
Published in final edited form as: J Gen Virol. 2005 Apr;86(Pt 4):1055–1065. doi: 10.1099/vir.0.80531-0

Fig. 2.

Fig. 2

Determination of Apo2L/TRAIL and TNF-α expression in DEN-2-infected HepG2 cells. HepG2 cells were infected with DEN-2 at an m.o.i. of 8 and cultured for the indicated time periods. (a) Apo2L/TRAIL, TNF-α and FasL mRNA expression in DEN-2-infected HepG2 cells analysed by RT-PCR. β-Actin mRNA was used as a control. C5/MJ and HUT-102 cells were used as positive controls (CON+) for Apo2L/TRAIL and TNF-α, respectively, and human T-cell leukaemia virus type I Tax-transfected Jurkat cells were used for FasL. (b) Supernatant from uninfected C6/36 cells and heat-inactivated DEN-2 does not upregulate Apo2L/TRAIL and TNF-α mRNA expression in HepG2 cells. (c) Apo2L/TRAIL expression in DEN-2-infected HepG2 cells cultured for 24 and 48 h, analysed by Western blotting. Protein extracts were separated by 10% SDS-PAGE, transferred to a membrane and blotted with either a specific anti-Apo2L/TRAIL mAb or an anti-actin antibody (as a protein loading control).