Fig. 6.

Induction of luciferase expression by Apo2L/TRAIL promoter in transfected HepG2 cells following infection with DEN-2. The pGL3 luciferase reporter construct containing the 5′-flanking region of Apo2L/TRAIL (nt −1056 to +86 relative to the transcription start site) or deletions of this region were transiently transfected into HepG2 cells, which were then mock infected with C6/36 supernatant or infected with DEN-2 (m.o.i. of 8) for 24 h, after which luciferase activity was assayed. Luciferase activities were normalized based on the Renilla luciferase activity from phRL-TK and expressed relative to cells transfected with ApoP/1056 followed by mock-infection, which were assigned a value of 1. Values represent the mean±SD of three independent transfections.