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. Author manuscript; available in PMC: 2010 Aug 6.
Published in final edited form as: Transplant Proc. 2009 Jun;41(5):1927–1931. doi: 10.1016/j.transproceed.2009.02.086

Figure 1. Allograft transplantation activates viral transcription.

Figure 1

Salivary gland and lung tissue lysates were evaluated by RT-PCR for viral transcriptional reactivation after allogeneic (H2b) skin grafting onto H2d mice latently infected with murine cytomegalovirus (MCMV) 21 days after transplantation. Both salivary glands and lungs showed transcriptional reactivation of late gene MCMV glycoprotein B (GB) in 3/4 mice. B. PCR analysis for MCMV DNA shows that the mouse “negative” for reactivation (lane 2) did not have detectable MCMV DNA, and subsequent antibody analysis showed that same mouse to have MCMV reactive antibody comparable to naïve mice (data not shown). B-Actin rows confirm adequate recovery of RNA or DNA respectively, and no-RT lanes confirm that RNA lack DNA contamination. Pos and Neg controls are technique controls.