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. Author manuscript; available in PMC: 2010 Aug 6.
Published in final edited form as: Transplant Proc. 2009 Jun;41(5):1927–1931. doi: 10.1016/j.transproceed.2009.02.086

Figure 2. Influence of cortisol on allograft triggered viral transcription.

Figure 2

Mice latently infected with murine cytomegalovirus (MCMV) received allograft (n=10) or isograft (n=4) skin. One half of allograft recipients (n=5) received concomitant cortisol, and another cohort (n=5) received cortisol alone (no skin graft). Twenty one days after grafting, RT-PCR analyses of salivary gland lysates show all allograft recipients reactivated latent MCMV (5/5), and that cortisol treatment abrogates allograft induced reactivation (0/5). Isograft skin or cortisol alone were insufficient to trigger latent MCMV. All graft recipients had MCMV DNA in their tissues, and all tested positive for MCMV reactive antibodies (not shown), confirming adequate MCMV infection. B-Actin rows confirm successful RNA recovery, and no-RT lanes confirm that RNA lack DNA contamination. Pos and Neg controls are technique controls.