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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Mol Microbiol. 2010 Feb 28;76(2):393–408. doi: 10.1111/j.1365-2958.2010.07103.x

Figure 2.

Figure 2

Schematic of the pCAEV1 and pΔospC strR vectors used for allelic-exchange replacement or gene deletion mutagenesis of ospC. A master construct, designated as pCAEV1, was generated to allow for the replacement of B. burgdorferi B31-5A4 type A ospC with ospC site-directed mutants. Panel A presents a schematic of pCAEV1 with a representative ospC gene inserted. Panel B presents a schematic of the pΔospC strR plasmid used to delete ospC from B. burgdorferi B31. The vectors were constructed as described in the text.