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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Mol Microbiol. 2010 Feb 28;76(2):393–408. doi: 10.1111/j.1365-2958.2010.07103.x

Figure 3.

Figure 3

Transgenic B. burgdorferi produce and present OspC at the cell surface. Control or transgenic strains of B. burgdorferi B31-5A4(indicated above each lane) were incubated with or without proteinase K, fractionated by SDS-PAGE, transferred to membranes and screened with anti-OspC or anti-FlaB antiserum (as indicated in Panel A). Panel B presents the results of immunofluorescent assays in which B. burgdorferi wild type or transgenic strains (indicated above each panel) were screened with anti-OspC antiserum. Methods were as described in the text.